This unique book on super-resolution microscopy techniques offers in-depth analyses of the strengths and weaknesses of various approaches. It is designed for non-experts seeking to grasp super-resolution principles or utilize recently commercialized instruments, as well as for professionals aiming to develop novel microscopic devices. The text covers practical requirements regarding hardware, software, and sample preparation, providing hands-on tips and tricks for setting up experiments, acquiring data, and analyzing and visualizing results. It spans a wide range of transdisciplinary applications. The author discusses the collaborative advancements in single-molecule photo-physics, fluorophore design, and instrument development that have enabled super-resolution microscopy. Subsequent chapters compare key techniques, including structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy, and multi-scale imaging methods like light-sheet and expansion microscopy. Each technique is introduced with experimental setups, imaging protocols, and application illustrations. The book concludes by addressing future challenges related to routine applications and the commercialization of methods. It serves as a comprehensive resource for researchers in applied sciences, including chemistry, biology, medicine, physics, and engineering, guiding users in designing their own experiments from the g
