This book describes a powerful and effective tool for the molecular biologist researcher. Readers will discover how DNA chromatography can be used in their own work. Intended as an introduction for the molecular biologist reader, this will also serve as a practical guide for the experienced user. The clear and concise writing style is also attractive to the chemist who wants to learn more about molecular biology.
Sample Preparation, Extraction, Chromatography
This first book on the market covers the many new and important RNA species discovered over the past five years, explaining current methods for the enrichment, separation and purification of these novel RNAs. Building up from general principles of RNA biochemistry and biophysics, this book addresses the practical aspects relevant to the laboratory researcher throughout, while discussing the performance and potential problems of the methods discussed. An appendix contains a glossary with the important terms and techniques used in RNA analysis. By explaining the basic and working principles of the methods, the book allows biochemists and molecular biologists to gain much more expertise than by simply repeating a pre-formulated protocol, enabling them to select the procedure and materials best suited to the RNA analysis task at hand. As a result, they will be able to develop new protocols where needed and optimize and fine-tune the general purpose standard protocols that come with the purification equipment and instrumentation.